Scooby-Doo! Mystery Incorporated is the eleventh incarnation of Hanna-Barbera's Scooby-Doo animated series, and the first incarnation not to be first-run on Saturday mornings. The series is produced by Warner Bros. Animation and Cartoon Network and premiered in the United States on Cartoon Network on April 5, 2010, with the next twelve episodes continuing, and the first episode re-airing, on July 12, 2010. The series concluded on April 5, 2013 with two seasons and fifty-two episodes, with a total of twenty-six episodes per season. Mystery Incorporated returns to the early days of Scooby and the gang, when they are still solving mysteries in their home town, though it makes many references to previous incarnations of the franchise, not least among them many cases and creatures from the original Scooby-Doo, Where Are You!. Episode by episode, the series takes a tongue-in-cheek approach to the classic Scooby-Doo formula, with increasingly outlandish technology, skills and scenarios making up each villain's story, and a different spin on the famous "meddling kids" quote at the end of every episode. Contrasting sharply with this, however, are two elements that have never been used in a Scooby-Doo series before: a serial format with an ongoing story arc featuring many dark plot elements that are treated with near-total seriousness, and ongoing relationship drama between the characters.

John Bennett, a man whose childhood wish of bringing his teddy bear to life came true, now must decide between keeping the relationship with the bear or his girlfriend, Lori.

Agent Jay is sent to find Agent Kay and restore his memory after the re-appearance of a case from Kay's past.